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hil 10  (R&D Systems)


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    R&D Systems hil 10
    Hil 10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+hil+10/Recombinant+Human+IL-10+Protein/pm40945404-80-8-22
    Average 94 stars, based on 59 article reviews
    hil 10 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: An IL-10 homologue encoded by human cytomegalovirus is linked with the viral "footprint" in clinical samples.
    Article Snippet: Persistent infections with human cytomegalovirus (HCMV) affect the hosts’ immune system and have been linked with chronic inflammation and cardiovascular disease.. These effects may be influenced by a HCMV-encoded homologue of the anti-inflammatory cytokine, IL-10 (cmvIL-10).. To assess this, we quantitated cmvIL-10 in plasma from renal transplant recipients (RTR) and healthy adults.

    Article Title: Human cytomegalovirus IL‐10 augments NK cell cytotoxicity
    Article Snippet: Immunology and Infectious Diseases Program, Division of BioMedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John’s, Newfoundland and Labrador, Canada Correspondence Dr.MichaelGrant,H1803 Immunology, Faculty ofMedicine,MemorialUniversity ofNewfoundland, 300PrincePhillipDrive, St. John’s,NL,A1B 3V6,Canada E-mail:mgrant@mun.ca Abstract Human cytomegalovirus (HCMV) persistently infects most of the adult population with periods of productive and latent infection differentially orchestrated by multiple HCMV-encoded gene products.. OneHCMVgene (UL111a) encodes cmvIL-10, a virokine homologous to human IL (hIL)10.. Although the effects of cmvIL-10 on most human lymphocyte subsets have been extensively studied, its impact on NK cell function was unreported prior to this study.We investigated effects of short-term cmvIL-10 exposure on human NK cells and found it substantially enhanced NK cell cytotoxicity through natural cytotoxicity receptors NKp30 and NKp46 as well as through Ctype lectin-like receptors NKG2C and NKG2D.

    Article Title: Human Cytomegalovirus Suppresses Type I Interferon Secretion by Plasmacytoid Dendritic Cells through Its Interleukin 10 Homolog
    Article Snippet: For induction of type I IFN expression, PBMCs (plated at a concentration of 1 × 10 7 cells/ml) or isolated PDCs (plated at a concentration of 5 × 10 5 cells/ml) were activated with 5 or 50 μg/ml A-class CpG oligodeoxynucleotides (ODN 2336, Coley Pharmaceutical Group), 5 μg/ml imiquimod (R837, InvivoGen), or heat-inactivated influenza virus A/Mem/71 equivalent to a MOI of 0.01. .. For IL-10 treatment, recombinant hIL-10 or cmvIL-10 (both from R&D Systems) was added to cultures concomitantly with the appropriate stimuli. .. Quantitative real-time PCR analysis Total RNA from PDCs was isolated using the RNeasy Mini kit (Qiagen) after treatment for 18 h. RNA processing and cDNA synthesis were performed as described previously ( Chang et al., 2004 ).

    Article Title: Antibodies to human IL-10 neutralize ebvIL-10-mediated cytokine suppression but have no effect on cmvIL-10 activity
    Article Snippet: .. Purified recombinant hIL-10, IFNγ, cmvIL-10, or ebvIL-10 (R&D Systems) were diluted to 10 μg/ml in PBS and analyzed by SDS-PAGE, transferred to a PVDF membrane, blocked, ... Of the seven antibodies that recognized hIL-10, four of these (JES3-9D7, JES3-12G8, JES3-19F1, and E43) also detected ebvIL-10. ..

    Article Title: IL-10 exacerbates xenogeneic GVHD by inducing massive human T cell expansion
    Article Snippet: .. To test IL-10 responsiveness single cell suspensions prepared from spleen were treated with 100 ng/ml of recombinant hIL-10 (R&D systems) for 15 minutes before staining for CD3, CD4, CD8 and pSTAT3. .. To measure serum levels of IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ and TNF-α human Th1/Th2/Th17 Cytokine Bead Array (BD Pharmingen, San Diego, CA) was used according to manufactures instructions.

    Article Title: Exposure of myeloid dendritic cells to exogenous or endogenous IL-10 during maturation determines their longevity.
    Article Snippet: A-class CpG oligodeoxynucleotides (50 g/ml; ODN 2336; Coley Pharmaceutical Group), imiquimod (5 g/ml; R837; InvivoGen), or heat-inactivated influenza virus (A/Mem/71) equivalent to a multiplicity of infection of 5 were used to activate PDCs. .. For IL-10 treatment, 5 ng/ml recombinant hIL-10 or cmvIL-10 (R&D Systems) were added to the cultures concomitantly with DC stimuli. ..

    Purification:

    Article Title: Antibodies to human IL-10 neutralize ebvIL-10-mediated cytokine suppression but have no effect on cmvIL-10 activity
    Article Snippet: .. Purified recombinant hIL-10, IFNγ, cmvIL-10, or ebvIL-10 (R&D Systems) were diluted to 10 μg/ml in PBS and analyzed by SDS-PAGE, transferred to a PVDF membrane, blocked, ... Of the seven antibodies that recognized hIL-10, four of these (JES3-9D7, JES3-12G8, JES3-19F1, and E43) also detected ebvIL-10. ..

    Membrane:

    Article Title: Antibodies to human IL-10 neutralize ebvIL-10-mediated cytokine suppression but have no effect on cmvIL-10 activity
    Article Snippet: .. Purified recombinant hIL-10, IFNγ, cmvIL-10, or ebvIL-10 (R&D Systems) were diluted to 10 μg/ml in PBS and analyzed by SDS-PAGE, transferred to a PVDF membrane, blocked, ... Of the seven antibodies that recognized hIL-10, four of these (JES3-9D7, JES3-12G8, JES3-19F1, and E43) also detected ebvIL-10. ..

    Staining:

    Article Title: IL-10 exacerbates xenogeneic GVHD by inducing massive human T cell expansion
    Article Snippet: .. To test IL-10 responsiveness single cell suspensions prepared from spleen were treated with 100 ng/ml of recombinant hIL-10 (R&D systems) for 15 minutes before staining for CD3, CD4, CD8 and pSTAT3. .. To measure serum levels of IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ and TNF-α human Th1/Th2/Th17 Cytokine Bead Array (BD Pharmingen, San Diego, CA) was used according to manufactures instructions.

    Single Cell:

    Article Title: IL-10 exacerbates xenogeneic GVHD by inducing massive human T cell expansion
    Article Snippet: .. To test IL-10 responsiveness single cell suspensions prepared from spleen were treated with 100 ng/ml of recombinant hIL-10 (R&D systems) for 15 minutes before staining for CD3, CD4, CD8 and pSTAT3. .. To measure serum levels of IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ and TNF-α human Th1/Th2/Th17 Cytokine Bead Array (BD Pharmingen, San Diego, CA) was used according to manufactures instructions.



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    A, B Data were normalised using the control group and are represented as fold‐change (FC) of the mean fluorescence intensity (MFI). Macrophage cells were obtained from human donors ( n = 4 biological replicas) and incubated with medium (CON) or recombinant human IL‐10 (hIL‐10r), MPN wild‐type (WT) or MPN expressing IL‐10 ORF (WT_IL10). Data are represented as mean ± standard error of the mean (SEM). Statistical analysis was performed using one‐way ANOVA + post hoc Tukey's multiple comparison test (* P < 0.05; ** P < 0.001, *** P < 0.0001). Source data are available online for this figure.

    Journal: Molecular Systems Biology

    Article Title: Bacterial expression of a designed single‐chain IL ‐10 prevents severe lung inflammation

    doi: 10.15252/msb.202211037

    Figure Lengend Snippet: A, B Data were normalised using the control group and are represented as fold‐change (FC) of the mean fluorescence intensity (MFI). Macrophage cells were obtained from human donors ( n = 4 biological replicas) and incubated with medium (CON) or recombinant human IL‐10 (hIL‐10r), MPN wild‐type (WT) or MPN expressing IL‐10 ORF (WT_IL10). Data are represented as mean ± standard error of the mean (SEM). Statistical analysis was performed using one‐way ANOVA + post hoc Tukey's multiple comparison test (* P < 0.05; ** P < 0.001, *** P < 0.0001). Source data are available online for this figure.

    Article Snippet: Control animals were processed in parallel and treated with (i) 2 μg of human IL‐10 recombinant protein (hIL‐10r) (PeproTech), or (ii) sterile PBS (vehicle solution).

    Techniques: Control, Fluorescence, Incubation, Recombinant, Expressing, Comparison

    A, B Detection of phosphorylated Tyr 705 of STAT3 (p‐STAT3) and unphosphorylated STAT3 by Western blot (see ). (A) Data from macrophages isolated from two independent donors, showing unstimulated cells (control, CON) or cells incubated for 24 h with human IL‐10 recombinant protein (hIL‐10r), supernatant of MPN WT (WT) or of MPN expressing IL‐10 (WT_IL‐10 ORF). (B) Data from HAFTL murine B‐cell line after exposure for 20 min to the supernatant of MPN WT (WT) or MPN expressing IL‐10 (WT_IL‐10 ORF). C HEK‐Blue™ reporter cell activation dose–response analysis by MutSC1 (linker NGGLD) and MutSC1_Gly (linker GGGGG) supernatants. The x ‐axis shows the range of IL‐10 concentration analysed (Molar, M), and the y ‐axis represents the mean ± SD of the absorbance at 630 nm. Data were generated in three independent assays with two technical replicas ( n > 6). D Western blot of p‐STAT3 activation after 20 min of induction with a fixed IL‐10 concentration (20 ng/ml) of supernatants from MPN WT (WT) or MPN expressing IL‐10 WT (WT_IL10 ORF), MutSC1 (WT_MutSC1), MutSC2 (WT_MutSC2) in two different cell lines: THP‐1 (human monocyte) and HAFTL (murine pre‐B cell line).

    Journal: Molecular Systems Biology

    Article Title: Bacterial expression of a designed single‐chain IL ‐10 prevents severe lung inflammation

    doi: 10.15252/msb.202211037

    Figure Lengend Snippet: A, B Detection of phosphorylated Tyr 705 of STAT3 (p‐STAT3) and unphosphorylated STAT3 by Western blot (see ). (A) Data from macrophages isolated from two independent donors, showing unstimulated cells (control, CON) or cells incubated for 24 h with human IL‐10 recombinant protein (hIL‐10r), supernatant of MPN WT (WT) or of MPN expressing IL‐10 (WT_IL‐10 ORF). (B) Data from HAFTL murine B‐cell line after exposure for 20 min to the supernatant of MPN WT (WT) or MPN expressing IL‐10 (WT_IL‐10 ORF). C HEK‐Blue™ reporter cell activation dose–response analysis by MutSC1 (linker NGGLD) and MutSC1_Gly (linker GGGGG) supernatants. The x ‐axis shows the range of IL‐10 concentration analysed (Molar, M), and the y ‐axis represents the mean ± SD of the absorbance at 630 nm. Data were generated in three independent assays with two technical replicas ( n > 6). D Western blot of p‐STAT3 activation after 20 min of induction with a fixed IL‐10 concentration (20 ng/ml) of supernatants from MPN WT (WT) or MPN expressing IL‐10 WT (WT_IL10 ORF), MutSC1 (WT_MutSC1), MutSC2 (WT_MutSC2) in two different cell lines: THP‐1 (human monocyte) and HAFTL (murine pre‐B cell line).

    Article Snippet: Control animals were processed in parallel and treated with (i) 2 μg of human IL‐10 recombinant protein (hIL‐10r) (PeproTech), or (ii) sterile PBS (vehicle solution).

    Techniques: Western Blot, Isolation, Control, Incubation, Recombinant, Expressing, Activation Assay, Concentration Assay, Generated

    Each point in the figure is a biological replica. A EC‐50 (molar, M) for human IL‐10 recombinant protein (hIL‐10r) and IL‐10 WT (IL‐10 ORF) and different variants (MutM, hIL‐10r, Mut1, Mut2, Mut3, MutSC1 and MutSC2) expressed by M. pneumoniae . Data are represented as mean ± SD. Statistical comparison was done by one‐way ANOVA + post hoc Bonferroni multiple comparison test, using the IL‐10 ORF condition as a reference (* P < 0.05). B Fold‐change (FC) in expression levels (fg/CFU) of IL‐10 variants Mut3 and MutSC1 secreted to the medium normalised by expression level of IL‐10 ORF (ORF). Statistical comparison of mean ± SD was performed by one‐way ANOVA + Tukey's post hoc test (* P < 0.05). C, D Average and SD values for the FC in the relative EC‐50 values determined by flow cytometry analysis of phosphorylated STAT3 after a 20‐min exposure of the BlaER1 (C) or HAFTL (D) cell lines to IL‐10 ORF (reference) or the mutant MutSC1 or MutSC2 (see ). Numbers indicate the average FC ± SD. Source data are available online for this figure.

    Journal: Molecular Systems Biology

    Article Title: Bacterial expression of a designed single‐chain IL ‐10 prevents severe lung inflammation

    doi: 10.15252/msb.202211037

    Figure Lengend Snippet: Each point in the figure is a biological replica. A EC‐50 (molar, M) for human IL‐10 recombinant protein (hIL‐10r) and IL‐10 WT (IL‐10 ORF) and different variants (MutM, hIL‐10r, Mut1, Mut2, Mut3, MutSC1 and MutSC2) expressed by M. pneumoniae . Data are represented as mean ± SD. Statistical comparison was done by one‐way ANOVA + post hoc Bonferroni multiple comparison test, using the IL‐10 ORF condition as a reference (* P < 0.05). B Fold‐change (FC) in expression levels (fg/CFU) of IL‐10 variants Mut3 and MutSC1 secreted to the medium normalised by expression level of IL‐10 ORF (ORF). Statistical comparison of mean ± SD was performed by one‐way ANOVA + Tukey's post hoc test (* P < 0.05). C, D Average and SD values for the FC in the relative EC‐50 values determined by flow cytometry analysis of phosphorylated STAT3 after a 20‐min exposure of the BlaER1 (C) or HAFTL (D) cell lines to IL‐10 ORF (reference) or the mutant MutSC1 or MutSC2 (see ). Numbers indicate the average FC ± SD. Source data are available online for this figure.

    Article Snippet: Control animals were processed in parallel and treated with (i) 2 μg of human IL‐10 recombinant protein (hIL‐10r) (PeproTech), or (ii) sterile PBS (vehicle solution).

    Techniques: Recombinant, Comparison, Expressing, Flow Cytometry, Mutagenesis

    Journal: Molecular Systems Biology

    Article Title: Bacterial expression of a designed single‐chain IL ‐10 prevents severe lung inflammation

    doi: 10.15252/msb.202211037

    Figure Lengend Snippet:

    Article Snippet: Control animals were processed in parallel and treated with (i) 2 μg of human IL‐10 recombinant protein (hIL‐10r) (PeproTech), or (ii) sterile PBS (vehicle solution).

    Techniques: Expressing, Recombinant, Control, Sequencing, Bicinchoninic Acid Protein Assay, RNA Extraction, Reverse Transcription, Amplification, Software, Imaging, Flow Cytometry, Mass Spectrometry, Real-time Polymerase Chain Reaction